Contact Us

BioSynTech Malaysia Group Sdn Bhd
199701004244 (419740-A)
No. 12A, Jalan TP 5,
Taman Perindustrian UEP,
47600 Subang Jaya,
Selangor, Malaysia

WhatsApp/Call: +6 016 216 0492
E : info@bstmgroup.com

Social Media

[MCE] RNA interference_ A Simple but Powerful Tool for Targeted Gene Silencing

15 Jul [MCE] RNA interference_ A Simple but Powerful Tool for Targeted Gene Silencing

Posted at 04:00h in News by
0 Likes


High-Quality Oligonucleotides for Effective RNA Interference

RNA Interference and Gene Silencing
RNA interference (RNAi) is a highly specific gene silencing mechanism mediated by RNA molecules. Unlike conventional inhibitors, RNAi offers unrestricted target sites, high specificity, and low toxicity. Key oligonucleotides in RNAi include small interfering RNA (siRNA), microRNA (miRNA), and antisense oligonucleotides (ASOs). These oligonucleotides bind to target mRNA sequences, inducing degradation or translational inhibition to silence genes[1].
miRNA-Based Tools
miRNA mimics: Synthetic double-stranded RNAs that can function like endogenous miRNAs they try to mimic.
miRNA inhibitors: Single-stranded oligonucleotides with complementary sequences that bind to target miRNAs and block their biological functions.
siRNA Applications
In vitro : MedChemExpress (MCE) provides pre-designed kits (human/mouse/rat) include three siRNA pairs per target, ensuring ≥70% mRNA knockdown (transfection efficiency >80%).
In vivo : Requires chemical modifications for optimal results. Custom siRNAs modifications (e.g., GalNAc conjugation) enhance stability for animal studies[2].
Antisense Oligonucleotides (ASOs)
ASOs target RNA via complementary binding, regulating gene expression by splicing, transcription, and translation (Figure 1)[3]. Applications include mRNA knockdown[3], pre-mRNA
alternative splicing modulation[3], 5’ cap formation inhibition[3], and poly(A) tail regulation[4]. The cellular stability of ASO is often enhanced by chemical modifications (e.g., LNA (locked nucleic acid), PS (phosphorothioate)). MCE offers custom ASOs with stability-enhancing modifications.
Figure 1. Regulation of target transcripts by single-stranded ASOs[3].
MCE Provides 45,000+ Oligonucleotides for Customers
Product Name Description
Patisiran
Sodium
 siRNA targeting transthyretin (TTR) mRNA

Inclisiran Sodium
 siRNA inhibits the transcription of PCSK9.

MicroRNA Mimic Negative Control
 21-nucleotide synthetic miRNA, negative control for
miRNA experiments

Fomivirsen Sodium
 21-mer phosphorothioate-modified ASO, targets
cytomegalovirus (CMV) RNA

Nusinersen Sodium
 SMN2 splicing modifier (spinal muscular atrophy)

Mipomersen Sodium
 Antisense oligonucleotide inhibitor of Apolipoprotein B
(apoB)

Inotersen Sodium
 Targets Transthyretin (TTR) transcript, reduces the
levels of the TTR protein.

Givosiran
 ALAS1-targeted siRNA (acute hepatic porphyria)

Bepirovirsen Sodium
 Antisense oligonucleotide targeting all HBV mRNAs.

Tofersen Sodium
 Targets superoxide dismutase 1 (SOD1) mRNA, reduces the
synthesis of SOD1 protein.
References:
[1] Weng Y, et al. Biotechnol Adv. 2019 Sep-Oct;37(5):801-825.
[2] Ebenezer O, et al. Int J Mol Sci. 2023 Feb 16;24(4):4019.
[3] Crooke, S.T., et al. Nat Rev Drug Discov. 2021 Jun;20(6):427-453.
[4] Yuan F, et al. Genes Dis . 2019 Oct 25;8(1):61-72.