CRISPR/Cas9 Knockout (KO) Plasmids consist of a pool of three plasmids each encoding the Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency
gRNA sequences are derived from the GeCKO (v2) library and direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
CRISPR/Cas9 KO Plasmids available for human and mouse genes are indicated by the (h) or (m) designation in the product name. Example: p53 CRISPR/Cas9 KO Plasmids (h) for human or p53 CRISPR/Cas9 KO Plasmids (m) for mouse
Provided as transfection-ready, purified plasmid DNA